| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072618 |
| Chromosome: | chromosome 17 |
| Location: | 360523 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g698365 | PF7,CCDC40,FAP172 | Flagellar Associated Protein 172 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATTTGGTTCAATTTGTGCTTCTTAAGTCCAATCAAAATTTCCGTGTAC |
| Internal bar code: | ACTTTATTTATACAATGTGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4087 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAGTGCACTTGACGCAAG |
| Suggested primer 2: | AAGCCATACAGCTCCACACC |