| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072625 |
| Chromosome: | chromosome 8 |
| Location: | 837748 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g361200 | CCDC151,ODA10 | Outer Arm Dynein Assembly protein 10 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTACAGTATTGCTTGTGCTATCATCATTATCATATAGTGTGCTACTATT |
| Internal bar code: | CCTTTACTAAAACAATGCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 983 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCCTTTACCCCTCCCCTCC |
| Suggested primer 2: | CGAATGGGTTGTGCACACAG |