| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.072634 |
| Chromosome: | chromosome 6 |
| Location: | 5827108 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g800706 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTACGCCGCGGCCGCGGCGCGAGGTGCTATTAGAACCCAGGAATGCTAC |
| Internal bar code: | ATTAAGCTAGTTACAAAGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 41.6667 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGACTGGGGCTGAGATGG |
| Suggested primer 2: | ACACAAACCAGCCTTGTCCA |