| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072640 |
| Chromosome: | chromosome 3 |
| Location: | 7712122 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g203650 | (1 of 1) IPR009072//IPR013785 - Histone-fold // Aldolase-type TIM barrel | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTACTGGCGCAAAGACTTACACGCACATCACGTACCATAAGATTCCT |
| Internal bar code: | GTCGGCGGAAATAACGATTGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3732 |
| LEAP-Seq percent confirming: | 99.1453 |
| LEAP-Seq n confirming: | 116 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 117 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCGATGGGGATCAGACTG |
| Suggested primer 2: | GGAGCAGCGCCCATACATAT |