| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072641 |
| Chromosome: | chromosome 17 |
| Location: | 1067077 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g703700 | SCLB1,SCL2,SUCLA2 | Succinate-CoA ligase beta chain; (1 of 1) K01900 - succinyl-CoA synthetase beta subunit (LSC2) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACCATCATCACCGCAGACGACCTGGACGACGCAGCCAAGAAGGCCGTG |
| Internal bar code: | ATAGTTAACCCATTTCTTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 393 |
| LEAP-Seq percent confirming: | 10.2041 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGCCACGACCTCTTCTT |
| Suggested primer 2: | GCATCATGAAGTGCGACGTC |