| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.072648 |
| Chromosome: | chromosome 5 |
| Location: | 1524549 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234661 | BCS1 | Ubiquinol-cytochrome c reductase complex chaperone; (1 of 1) K08900 - mitochondrial chaperone BCS1 (BCS1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGAGGCCCCGCATCTAGTGCGACAGCGCGTCTCAAATGGAGATTACT |
| Internal bar code: | CGGCTAAGTTGAGCGGGTGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1320 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGAAGCCCAAGGGAAAGAT |
| Suggested primer 2: | CTTGACGATCGGGATGGGAG |