Insertion junction: CLIP2.072648_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.072648
Chromosome:chromosome 11
Location:1504917
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCTCTCCATCACACACCCCACGACACCTCCAGTTCTCTACAGATAAGCT
Internal bar code:GGCATTGATCAAGCCTTTATAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2042
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:48
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:48

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGATAGTTGGTCGCTCG
Suggested primer 2:CTGCCTTGCTGCTTCTGTTG