| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072650 |
| Chromosome: | chromosome 10 |
| Location: | 4348472 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g449850 | (1 of 274) IPR020683 - Ankyrin repeat-containing domain | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAAGCCTTACTTAAATCTTTAAGGAAACACGCAGCTCGCGCATTCTCA |
| Internal bar code: | CAGCTAACAGTTTGTCCACTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1520 |
| LEAP-Seq percent confirming: | 82.7957 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCAGGGTGTTCGTTCT |
| Suggested primer 2: | CAAACACCCAATGCTGCCTC |