| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072651 |
| Chromosome: | chromosome 17 |
| Location: | 6891873 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g747697 | mS33,MRPS33 | Mitochondrial ribosomal protein S33 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAGTACCTCCTCAATAGTCGCTGCACAAGTGTCGGCCCGCCGGCCGCT |
| Internal bar code: | AGTTTGTGCCATCTACGCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2805 |
| LEAP-Seq percent confirming: | 96.7742 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGAGGGGATGTATGCTC |
| Suggested primer 2: | GTGTGCGCATAGTAAGCTGC |