| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072655 |
| Chromosome: | chromosome 8 |
| Location: | 2076153 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g369200 | (1 of 2) IPR000104//IPR000719//IPR002290//IPR011009 - Antifreeze protein, type I // Protein kinase domain // Serine/threonine/dual specificity protein kinase, catalytic domain // Protein kinase-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTGTTGAAGTCCAGCAGCTGCTGGGCGCCGCCCGGAGGCTGCGGCTGC |
| Internal bar code: | TGGGAATCACTAGTGTAGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGAGTGCCATGATGGTGAC |
| Suggested primer 2: | GGGATGGATGAACACGCAGA |