| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072657 |
| Chromosome: | chromosome 10 |
| Location: | 3100970 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g441450 | (1 of 1) K14786 - protein KRI1 (KRI1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGAGGAGCCGGGCGCGGACCGCATCATCACGCACCCCCGTAACGTGGA |
| Internal bar code: | CACTTACATCAGGGGTTCTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCTCACGTCGTAGTAGTC |
| Suggested primer 2: | GGTGTGGTGTAGGTACGTGG |