Insertion junction: CLIP2.072676_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.072676
Chromosome:chromosome 17
Location:2415220
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g714500 HTA33,NCG10,HTA1 Histone H2A; (1 of 30) K11251 - histone H2A (H2A) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAATTTCATTCCCACTTGCACTCAATCAACAAACTCGGTGTTTCTCAACA
Internal bar code:GACTTGGATGTACAATGTTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:3034
LEAP-Seq percent confirming:93.617
LEAP-Seq n confirming:44
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:CATGGTGCTCAGGATTGGGT
Suggested primer 2:GAGCGGTTGTTTGACCCAAC