| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072678 |
| Chromosome: | chromosome 13 |
| Location: | 1445386 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572100 | 5'UTR | ||
| Cre13.g572150 | NAT29 | N-acetyltransferase; (1 of 1) PTHR14744:SF15 - N-ALPHA-ACETYLTRANSFERASE 60 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGCTTTATATCGGCGCTGCGCAAAGTAAAGGGTGTTTTCTGCACTAGG |
| Internal bar code: | ATGCGTGCTGGGGCCGATTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1028 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCGGGTTCCTTGAAACC |
| Suggested primer 2: | TCAACGGAAGCAGCTACAGG |