| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.072685 |
| Chromosome: | plastome |
| Location: | 193344 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802334 | orf528 | unknown ORF | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATATAAAAAAATAATCAAACACACTTTTTTTAAATTATTTGTAAAAAA |
| Internal bar code: | ATAGTGCATAGTAAGTGGACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 43 |
| LEAP-Seq percent confirming: | 4.34783 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGTTAAGCTCGAACCCC |
| Suggested primer 2: | ACGTCAGTGGCTGTTGAAGT |