| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072689 |
| Chromosome: | chromosome 3 |
| Location: | 6880436 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197850 | (1 of 2) PF11267 - Domain of unknown function (DUF3067) (DUF3067) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAATGAAAGCTAGTGTGAATGCAAATGCAAGACGCCATCGAGTGCTCCA |
| Internal bar code: | TCCATTTGGGATCCCGAATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6485 |
| LEAP-Seq percent confirming: | 83.8095 |
| LEAP-Seq n confirming: | 88 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACGGCGTGATTGAAATC |
| Suggested primer 2: | TGCTACACTAGGAGCGAGGT |