Insertion junction: CLIP2.072697_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.072697
Chromosome:chromosome 12
Location:2659902
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g505400 (1 of 1) 3.1.1.47 - 1-alkyl-2-acetylglycerophosphocholine esterase / Platelet-activating factor acetylhydrolase; Related to platelet-activating factor acetylhydrolase outside_mRNA
Cre12.g505450 HFO17 (1 of 33) K11254 - histone H4 (H4); Histone H4 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATTTCCCCAGACTCCATTTCCTCGACCCCGACCCCAACCCCTCCCCGG
Internal bar code:ATTCGTGTTTAGCGCCGAAGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:3077
LEAP-Seq percent confirming:96.7213
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:61

Suggested primers for confirmation by PCR

Suggested primer 1:ATTACCAAGCCCGCTATCCG
Suggested primer 2:AGTGCGTGAGACGACAACAT