| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072707 |
| Chromosome: | chromosome 10 |
| Location: | 4775645 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452850 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGTTGGTGAAGTCCCACAGGTTGCAGCTGATTCCCAGGCCCCAGCAG |
| Internal bar code: | ACATCAAGCGACTATTAGCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 729 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTAACGTGGGACAACGTG |
| Suggested primer 2: | TCCTCGTCAGGAGTCGATGT |