| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.072712 |
| Chromosome: | chromosome 8 |
| Location: | 1269116 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g363800 | (1 of 1) PTHR31996:SF2 - COILED-COIL DOMAIN-CONTAINING PROTEIN 115 | 5'UTR | |
| Cre08.g363837 | POLG1X,POL1A | (1 of 1) IPR001098//IPR002298//IPR012337 - DNA-directed DNA polymerase, family A, palm domain // DNA polymerase A // Ribonuclease H-like domain; Putative DNA polymerase gamma, organellar | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCATGGCAACCTATTGGCAATGATGACATCAGAAAAAGTTAAGTCG |
| Internal bar code: | TGCGCTGGCGCTAGGGCGATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 130 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCTGATCCAGATCCGGA |
| Suggested primer 2: | TCATCGTTAGAGAGCGTGGC |