| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.072713 |
| Chromosome: | chromosome 4 |
| Location: | 4078551 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g233302 | (1 of 2) PTHR23132//PTHR23132:SF9 - D-ALANINE--D-ALANINE LIGASE // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGACGAAGTGTATGGGGAAACCATCTAATCAGGATGTAAGTAAGTGTCG |
| Internal bar code: | CGGTCCGTCTTTTGGGCGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1176 |
| LEAP-Seq percent confirming: | 86.6667 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACACTTGCCCTGAACACG |
| Suggested primer 2: | TACATCGTCTACCCTGGGCA |