| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072720 |
| Chromosome: | chromosome 17 |
| Location: | 1186723 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g704550 | HRF1 | (1 of 1) PTHR14083:SF0 - PROTEIN YIF-1, ISOFORM B; HRF1 homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTGTTGTGCTGTCGGCGCGTTGGTCGTTGCGCGGGCGCCTATGCGCCC |
| Internal bar code: | TTATTTAGTATTTCTTCCCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1489 |
| LEAP-Seq percent confirming: | 92.8571 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCTCCTCCTCTTCAGGC |
| Suggested primer 2: | CGTCAGCCATGCCTAATTGC |