| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072725 |
| Chromosome: | chromosome 7 |
| Location: | 478904 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315550 | (1 of 2) IPR005654//IPR027417 - ATPase, AFG1-like // P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTGTAAATAATTCATCAAGTTGCCCTCGCGTGCGTACTCGGGACACCG |
| Internal bar code: | GAATGGTACGACCGTCTCAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1687 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGTCGTGCACATTGCTG |
| Suggested primer 2: | AGTACCCAGCAGCATCCCTA |