| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072726 |
| Chromosome: | chromosome 7 |
| Location: | 2645463 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g330550 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGGAGGTTTGGGTGCCCGGATGACCTGGGCCAATAGACAGTCATGCG |
| Internal bar code: | CGTCGTAGAATTATTAGGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2754 |
| LEAP-Seq percent confirming: | 60.7143 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAAGGCAGCCAATCCTCC |
| Suggested primer 2: | GCAGAGCGATTGTGATGCAG |