| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072727 |
| Chromosome: | plastome |
| Location: | 20751 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802271 | ChreCp009,2717052,chlL | light-independent protochlorophyllide reductase iron-sulfer ATP-binding protein; (1 of 1) K04037 - light-independent protochlorophyllide reductase subunit L (chlL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAAACTCCATCGGAAGATTATATTTGTAATTTTTATTTAAATATTGCT |
| Internal bar code: | GTATAAGTTTGTGTGCGATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 707 |
| LEAP-Seq percent confirming: | 54.5455 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCAGCCAACAAATGCCA |
| Suggested primer 2: | ACGGAGGTTATGGGGGTGTA |