| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072728 |
| Chromosome: | chromosome 10 |
| Location: | 4085854 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448200 | ARL9 | ARF-like GTPase; (1 of 4) K07937 - ADP-ribosylation factor 1 (ARF1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGTAACGCTTTGTTTAAAGCGTGACAGCTGAAGTAGATCAACATGCA |
| Internal bar code: | TTCTGTGCGGTGACGTCGATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2026 |
| LEAP-Seq percent confirming: | 78.5714 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTTGTGTTGCAGAGTG |
| Suggested primer 2: | TGCGTTTGCTGTGCCTACTA |