| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072734 |
| Chromosome: | mating type plus |
| Location: | 6369 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g252250 | MTP0428 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAGGCCGCGCATCCGTCAACACCCCCTCCTCACTGGCAGCACCAGC |
| Internal bar code: | TCCTTGTTCCGGACGCAGTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3096 |
| LEAP-Seq percent confirming: | 26.3158 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTGCATGGTAAGTTCGGCA |
| Suggested primer 2: | AGCGGTAGGTGTTTCAGGTG |