| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072738 |
| Chromosome: | chromosome 12 |
| Location: | 8862257 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g546350 | GEX157 | (1 of 4) PF05653 - Magnesium transporter NIPA (Mg_trans_NIPA) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCGTTTAGAAACTACGCATCTGCTGTCAAGAGCGGTCTTGATACTGC |
| Internal bar code: | TCAGCGCGAATTCTGGGGCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3018 |
| LEAP-Seq percent confirming: | 96.5517 |
| LEAP-Seq n confirming: | 84 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTTTTTGGCCATGCTGA |
| Suggested primer 2: | ATTTAGGCCTCGGTACCCCT |