Insertion junction: CLIP2.072759_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.072759
Chromosome:chromosome 12
Location:3669342
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g513550 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACTTGTGTGGTAACTCTGTGTCATATACTCTGTGTCATATACTCTGT
Internal bar code:CCTGTATTTGTCCGCAACAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:4778
LEAP-Seq percent confirming:98.3333
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACTTCTACCCCCTGCAC
Suggested primer 2:GAGCAGGACAACGAGAAGCT