Insertion junction: CLIP2.072761_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.072761
Chromosome:plastome
Location:10693
Confidence (%):40
Locus systematic id Locus common name Defline Feature
CreCp.g802266 2716978,psbK,ChreCp004 (1 of 1) K02712 - photosystem II PsbK protein (psbK); photosystem II K protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTAATTTTTTAGCCTGTTAATATCCTTCTAGGAGGATAGCAGTTGACAG
Internal bar code:CGTACGGGTCATTGTAAACATT

Confirmation - LEAP-Seq

LEAP-Seq distance:262
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:ACCAACTGAACCACCAGCTG
Suggested primer 2:CATAGGGTCGCAGGTTCGAA