| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072763 |
| Chromosome: | chromosome 12 |
| Location: | 3479443 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g497150 | 3'UTR | ||
| Cre12.g497200 | FAP216 | EF-Hand Flagellar Associated Protein 216 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGTGATACAACCAAGGTAGTTCCAATAGATATGTGGGGTTCCTGCAG |
| Internal bar code: | GAGGTGTTTCCAGTCCGAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6558 |
| LEAP-Seq percent confirming: | 94.0476 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATACGCGACACCACCAAG |
| Suggested primer 2: | TGGTTTGAGCGAGGCCTTAG |