| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072774 |
| Chromosome: | chromosome 10 |
| Location: | 2692323 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g437550 | 5'UTR | ||
| Cre10.g801134 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTACCTGCAAGACACCGCCGCGCAGCTGGGCATATCCGTGCGGCAGCAG |
| Internal bar code: | GAAAAGAGTGTTACGAGTTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 898 |
| LEAP-Seq percent confirming: | 78.125 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACCTCTCAAAGCTGCCG |
| Suggested primer 2: | GTGTGTTTACGCCGTGCTTT |