| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072778 |
| Chromosome: | chromosome 1 |
| Location: | 613689 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003524 | (1 of 1) PF13499//PF14531 - EF-hand domain pair (EF-hand_7) // Kinase-like (Kinase-like) | 5'UTR | |
| Cre01.g800006 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAATTATCAGTTAAGCGCGAAGCACGGCCTCGAGTGCAAAACTTGTTCT |
| Internal bar code: | TAAATTTTATAGAACTAGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1497 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTTCGGGCGTGTTTGTA |
| Suggested primer 2: | CCCATTTGTGAGGCAGGACT |