| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072779 |
| Chromosome: | chromosome 3 |
| Location: | 2003649 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155800 | (1 of 1) K15025 - probable RNA-binding protein EIF1AD (EIF1AD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCCCTGGCTGCAATCGCCCAAACAGCACCTGGGCGTAAACTTGCGTA |
| Internal bar code: | TTGCGGATGAAGGTCTCATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 871 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTAAACGCAGGCTCACTT |
| Suggested primer 2: | TCAGATCGAACCGATGCTCG |