| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072782 |
| Chromosome: | chromosome 12 |
| Location: | 399988 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g495350 | POC12 | (1 of 1) K19332 - Meckel syndrome type 1 protein (MKS1); Proteome of centriole protein 12 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGCGCATGGCTTTGCCCTCCTTTGATGAACGCTGCACGGCCGCCGGC |
| Internal bar code: | CTATTGTAATATAATGACTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 74 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAAGGAGTGTGTGACGCT |
| Suggested primer 2: | GGTTGTCAGTGTCACCGTCT |