Insertion junction: CLIP2.072784_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072784
Chromosome:chromosome 9
Location:2107377
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g396100 PHC15,EZY16 Pherophorin-chlamydomonas homolog 15; (1 of 71) PF12499 - Pherophorin (DUF3707) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCCCCATCCCCAACCCAGGTGTCTGGTCCAAGAAGGGCGACAGCTGGG
Internal bar code:CTGGGAGGAGGGCTTAGGCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:80
LEAP-Seq percent confirming:6.06061
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCCCGAACTTCAAAAACC
Suggested primer 2:CCAGTAACGCGGTTTTGGTG