| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072788 |
| Chromosome: | chromosome 3 |
| Location: | 4597833 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g176600 | FAP374 | Flagellar Associated Protein 374 | 3'UTR |
| Cre03.g176651 | MYSM1 | (1 of 1) PF00249//PF01398 - Myb-like DNA-binding domain (Myb_DNA-binding) // JAB1/Mov34/MPN/PAD-1 ubiquitin protease (JAB); Histone H2A deubiquitinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGAATGACTAGCAGCTAGCAAATCAGAGGCGGCGGTAGGCCGGTCGT |
| Internal bar code: | TGCTTGATTCGATCTGTCCAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1285 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTTAAGAATGTGGGCCGG |
| Suggested primer 2: | GCACATGCATAAGGAGCTGC |