| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072794 |
| Chromosome: | chromosome 6 |
| Location: | 5506515 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286500 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGACTGCAATCGGTAACCCTGCATATGCTCGGTATGATGCAAGCCCACGT |
| Internal bar code: | GTAAATGTAAGTGTGGTCACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1748 |
| LEAP-Seq percent confirming: | 90.6977 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCGTGTGTCTGTAGCATGT |
| Suggested primer 2: | TTCGCTTGTGTTCTAGGCGT |