| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072795 |
| Chromosome: | chromosome 12 |
| Location: | 454173 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g494800 | DII1,IDA-IC28,IDA4,p28IDA4 | (1 of 1) K10410 - dynein light intermediate chain, axonemal (DNALI); Flagellar inner arm dynein light chain p28 | 5'UTR |
| Cre12.g494850 | ADK3 | Adenylate kinase; (1 of 1) PTHR23359//PTHR23359:SF72 - NUCLEOTIDE KINASE // ADENYLATE KINASE FAMILY PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGCGATGTTGCGAGGGCGACGCTGCCATGCCAGCCCACTGGTCCACAT |
| Internal bar code: | AGACCATGCCGCGATTTGCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3818 |
| LEAP-Seq percent confirming: | 94.2308 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGTTGTGTTGTCAGGC |
| Suggested primer 2: | CATCAAACGTCTGCGCGTAG |