| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072813 |
| Chromosome: | chromosome 12 |
| Location: | 7750804 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g554400 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTTGCCGACACGCACGAGCAGAGGCCTCCCTCGCGGCCTCTCACAAA |
| Internal bar code: | TCGTATTGGGGATCATCCTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 993 |
| LEAP-Seq percent confirming: | 40.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGTCTGGCATTAGCAGTT |
| Suggested primer 2: | TGGCCTTTGCATTGGAAAGC |