| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072816 |
| Chromosome: | chromosome 12 |
| Location: | 6090007 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g535019 | (1 of 139) IPR029787 - Nucleotide cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACAGATGCTGCGATCCGATGTGATGGTAGCACGGTAGGGCGGGCGTCCG |
| Internal bar code: | CATTTGTTGTAGCTAAAGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4833 |
| LEAP-Seq percent confirming: | 96.7391 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAACTTCCCCTTTGCTTG |
| Suggested primer 2: | GCATCTCCATCCATCGAGCA |