| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072823 |
| Chromosome: | chromosome 3 |
| Location: | 3791616 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169700 | TMG11 | tRNA (guanosine-2'-O)-methyltransferase; (1 of 1) PTHR12029:SF45 - RRNA METHYLASE-LIKE PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTCCCGTCCGCCTTGTTCACCGCATGCACGGCACCGTAGCCCAGCGCT |
| Internal bar code: | GTACTGACGGTATGGTCCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 968 |
| LEAP-Seq percent confirming: | 20.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGTAGGGCCAGATGCT |
| Suggested primer 2: | ACCTCCTGGATGGTGATGGA |