| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.072825 |
| Chromosome: | chromosome 3 |
| Location: | 7650917 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g204250 | SAH1 | (1 of 1) 3.3.1.1 - Adenosylhomocysteinase / SAHase; S-Adenosyl homocysteine hydrolase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGCGGGAAGTGGCTTCCCAAGGGCCTGGCTTGTGCGTGTGTGGCTGCG |
| Internal bar code: | CTCTTTCGTGGTCACCTATACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTAGCTGCACCAGTCACA |
| Suggested primer 2: | CTCAACAAACACAAGCCCGG |