Insertion junction: CLIP2.072835_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072835
Chromosome:plastome
Location:24020
Confidence (%):40
Locus systematic id Locus common name Defline Feature
CreCp.g802274 2717055,ChreCp012,rpl2 50S ribosomal protein L2; (1 of 2) IPR005880 - Ribosomal protein L2, bacterial/organellar-type CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCATCCACGTGGTTTAAATATTGGTGATATTATTCAATCTGATTTAAAC
Internal bar code:CTTCAGGGAATACCATTCTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:784
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CATGGTGTAACTGGACGGCT
Suggested primer 2:AGGTGCCAATGCGTAGTTGA