| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.072837 |
| Chromosome: | chromosome 17 |
| Location: | 3553979 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g724873 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCCGCATGTCTGAGTGTTGCAGGCGTTATAACGGCCCCCCGGCCCCC |
| Internal bar code: | AATCTTGAAAGCAGTTCGGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 376 |
| LEAP-Seq percent confirming: | 28.0 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCAACGGTATTCCCAATC |
| Suggested primer 2: | CTCTTGCGTAGGGCTATGGG |