Insertion junction: CLIP2.072839_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.072839
Chromosome:plastome
Location:102103
Confidence (%):63
Locus systematic id Locus common name Defline Feature
CreCp.g802308 ChreCp044,2716995,psbL photosystem II reaction center subunit XII; (1 of 1) K02713 - photosystem II PsbL protein (psbL) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATACTGGGGTTTACTTTTAATTTTCGTATTAGCTGTTCTTTTCTCTAGTT
Internal bar code:TTTGCATCAATAGTGCGCTTTA

Confirmation - LEAP-Seq

LEAP-Seq distance:82
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCCGAAGAGACTACCAACA
Suggested primer 2:GCGGCTGTGGCTGTAGATAT