| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072853 |
| Chromosome: | chromosome 16 |
| Location: | 2882019 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g663100 | ALG7 | (1 of 1) 2.7.8.15 - UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase / N-acetylglucosamine-1-phosphate transferase; N-acetylglucosamine phosphostransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTCTTCTCTTGTTGTTCTGCAGCACCTGGTAGAATGTCGCGGGCGGTG |
| Internal bar code: | GCTGGTTCAATAAACGCACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 662 |
| LEAP-Seq percent confirming: | 3.84615 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGCAGATTACGCACAGC |
| Suggested primer 2: | TGGCCATGCAGCTACAATGA |