| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.072856 |
| Chromosome: | chromosome 6 |
| Location: | 5355664 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g285050 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGCATCGCTACGGTACCTGTATGCATCTTGATGACATGACGCGCTGCT |
| Internal bar code: | TCATCAGACCCGGACGTTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 456 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAATTTCAGGACCAGCCG |
| Suggested primer 2: | GCGTCGTATGTTCCATTGGC |