Insertion junction: CLIP2.072866_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072866
Chromosome:chromosome 4
Location:1910343
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre04.g218450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATACGGTAGATCTTACTCCCTGGGTGGGTGTGGAGTCCATCGCCGCTT
Internal bar code:AGGCAGCACTACGTCCGTCTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:286
LEAP-Seq percent confirming:10.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCATGCAAAACCAACCCC
Suggested primer 2:CGTGCGCATTATCACAGCAA