| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072884 |
| Chromosome: | chromosome 6 |
| Location: | 7355765 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299900 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCGCCCCCTAAATCCACTCCGCCACCAAATCCGCCGTCCCCTGACCC |
| Internal bar code: | TTTTAGGTAATTAACTGGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5599 |
| LEAP-Seq percent confirming: | 91.2 |
| LEAP-Seq n confirming: | 114 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 125 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGCAACAATGTCCACGT |
| Suggested primer 2: | TGTGAACGACTGCTCCTTCC |