| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072890 |
| Chromosome: | chromosome 10 |
| Location: | 2876303 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439600 | (1 of 1) PTHR11649//PTHR11649:SF62 - MSS1/TRME-RELATED GTP-BINDING PROTEIN // PROTEIN SEY1 HOMOLOG | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGCGGGAGGGGGTTGCCGACTCGGGGGGAGGGGGTTGCCGACGTCCA |
| Internal bar code: | TGTCACATATACACATTCCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 244 |
| LEAP-Seq percent confirming: | 77.7778 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTTATGGGCTTGGGACT |
| Suggested primer 2: | TTCCGTAGCACCTGCAGAAG |