| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.072905 |
| Chromosome: | chromosome 3 |
| Location: | 7262457 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g207502 | (1 of 7) K18932 - palmitoyltransferase (ZDHHC) | 3'UTR | |
| Cre03.g207550 | ADH8,CAD2 | Cinnamyl-alcohol dehydrogenase; (1 of 2) 1.1.1.195 - Cinnamyl-alcohol dehydrogenase / CAD | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAATTACGTGTAGCGGGTCAAGGCGCTGAAAGCAGAGGGGCCACGCCA |
| Internal bar code: | TATAAAGAGTCGGGCCTGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3241 |
| LEAP-Seq percent confirming: | 52.5 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAATCCAGCCCCATTGTCA |
| Suggested primer 2: | CCTGTAGCAGTGACGTTGGT |